Inducing Lipid Domains in Membranes by Self‐Assembly of DNA Origami

Self-assembly of biological molecules and structures is a fundamental property life. Whereas most functions are based on self-assembled proteins protein complexes, the self-assembly lipids important for spatial organization heterogeneous cellular reaction environments to catalyze cooperative interactions on/with membranes. Lipid domains or “rafts”, which known selectively recruit proteins, play an functional role in sorting trafficking membrane components between subcellular organelles. However, how recruitment turn contributes formation turnover these has not been systematically addressed, due large variety membrane–protein features their spatiotemporal dynamics. The small size transient nature lipid adds complexity visualizing them living cells. Here, DNA origami presented as programmable tool mimic clustering assembly membranes illustrate nanometer sized coalesce into visible upon defined patterns. Hence, local composition can be efficiently regulated by peripheral binders. This reinforces hypothesis that rafts cells occur result and, particular, self-assembly.